Cell Process

1147 words 5 pages
Cell Processes: Photosynthesis
Part 1: Pigments in Plant Leaves
Purpose: The purpose of this experiment is to visualize the pigments present in plant leaves by using paper chromatography.
Materials Used: Chromatography vial with cap, wax pencil, disposable transfer pipet, chromatography solvent, chromatography paper strip, sharp pencil, ruler, pair of scissors, piece of fresh pre-soaked spinach, coin, pair of forceps.
Observations and Data: Distance of Pigments From Original Pencil Line | | Pigment | Color | Migration Distance (mm) | Rf Value | | Carotene | Orange | 59 mm | .94 | | Xanthophyll | Yellow | 56 mm | .89 | | Chlorophyll a | Light Green | 29 mm | .46 | | Chlorophyll b | Dark Green | 14 mm | .22 |
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Materials Used: 0.2% sodium bicarbonate solution with dish soap added, sodium bicarbonate solution, disposable beaker 100 mL, one hole punch, timer, light source, 10 mL syringe, piece of fresh pre-soaked spinach.
Observations and Data: Rate of Photosynthesis in Leaves | Time (seconds) | Number of Floating Disks | 0 | 0 | 30 | 0 | 60 | 0 | 90 | 0 | 120 | 0 | 150 | 0 | 180 | 0 | 210 | 0 | 240 | 0 | 270 | 0 | 300 | 0 | 330 | 0 | 360 | 0 | 390 | 1 | 420 | 1 | 450 | 2 | 480 | 2 | 510 | 2 | 540 | 2 | 570 | 3 | 600 | 3 | 630 | 3 | 660 | 3 | 690 | 3 | 720 | 3 | 750 | 3 | 780 | 3 | 810 | 3 | 840 | 4 | 870 | 4 | 900 | 5 | 930 | 5 | 960 | 5 | 990 | 5 | 1020 | 5 | 1050 | 6 | 1080 | 8 | 1110 | 8 | 1140 | 7 | 1170 | 9 | 1200 | 9 | 1230 | 9 | 1260 | 10 |

Analysis and Results:
Refer to part 1 analysis and results.
Part 3: Rate of Photosynthesis in leaves with closer light resource
Purpose: The purpose of this experiment is to understand if there’s stronger light source that is presented to the leaves, does the leave photosynthesize faster and does it produce oxygen as by product faster.
Materials Used: 0.2% sodium bicarbonate solution with dish soap added, sodium bicarbonate solution, disposable beaker 100 mL, one hole punch, timer, light source, 10 mL syringe, piece of fresh pre-soaked spinach.

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