Enzyme Lab Report

1042 words 5 pages
AP Biology Lab: Catalase (Enzymes)

Abstract In this laboratory exercise, studies of enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes.
Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment.
Four AP Biology classes performed this experiment and the data presented in this report will reflect the averages of said classes. In the later discussion sections, it will become
…show more content…

Rate vs.pH 2A 3A 4A 4B Average
NaOH - 12 0.16 0.09 0.062 0.15 0.12
Malox - 9 0.22 0.13 0.057 0.13 0.13
Hydrogen Peroxide - 7 0.17 0.16 0.062 0.16 0.14
Vinegar - 3 0.1 0.19 0.051 0.11 0.11
HCL - 1 0.09 0.24 No Rx 0.1 0.108

The rate peaked at a pH of 7 and then decreases outward.

Rate vs. Temperature 2A 3A 4A 4B Average
0 degrees C 0.67 0.09 0.66 0.18 0.4
5 degrees C 1 0.13 0.84 0.13 0.46
25 degrees C 0.5 0.16 0.02 0.22 0.11
40 degrees C 0.22 0.19 0.19 0.1 0.18
60 degrees C 0.33 0.24 0.05 0.08 0.175

At the lower temperatures, the activity rate was high but then at 25oC, the rate dropped. After that the rate seems to be increasing toward another drop-off point.

Inhibitor Effects 2A 3A 4A 4B Average
Control 0.2 0.095 0.52 0.115 0.23
Hydroxylamine 0.0083 0.001 0.008 0.004 0.005

The inhibitor slowed the reaction to almost a complete stop.

Conclusion In conclusion, the classes have come to the result that each enzyme has a specific set of conditions that must be met for optimum activity. The changes in the temperature, pH, substrate concentration and enzyme concentration, cause fluctuations in the activity rate of the enzyme. Also, if the temperature or pH is too high or too low, then the

Related

  • Bio 101
    24972 words | 100 pages
  • Leaflab Report
    931 words | 4 pages
  • Ptc Taster Genomic Analysis Lab Report
    1183 words | 5 pages
  • Understanding Enzymes
    1606 words | 7 pages
  • Preeclampsia Case Study
    2038 words | 9 pages
  • Effect of Varying Temperatures on Enzymatic Activity of Bacterial and Fungal Amylase and Hydrolysis of Starch
    2755 words | 12 pages
  • Unknown Lab Report
    2134 words | 9 pages
  • Microbio lab report body
    2609 words | 11 pages
  • Patient Education Plan: Teaching the Patient with Hepatitis C
    1557 words | 7 pages
  • Role of Inflammation in Atherosclerosis
    8542 words | 35 pages