Phage Lab

1606 words 7 pages
Alisha Patel
Genetics Lab
TA: Vasant Chary
March 21, 2012

Phage Titering of a Bacterial Culture and Recombination of Bacteriophage

OBJECTIVES:

The main objectives of this experiment included making dilutions of solutions, plating phage or bacteria, and determining the number of bacterial viruses or phage in a suspension. It was also conducted to demonstrate that two different mutants of phage T4 can exchange genetic material to give rise to wild-type phage. The experiment was used to distinguish mutants from wild-type by their host specificity. The recombination in bacteriophage was performed to determine the concentration of unadsorbed phage from the U series plates, total concentration from B series, and concentration of
…show more content…
Mutant strains could only be formed on E. Coli B whereas wild-type phage was able to grow on both E. Coli K and E. Coli B. Both mutations are part of the same cistron in the rII regions.
E. Coli B is infected multiple times with both mutants. Phenotypically, the appearance of plaques on E. Coli B in the parents for both phages was large whereas there were no plaques on E. Coli K. The recombinants for double mutant were large while the wild-type was small in E. Coli B whereas in E. Coli K, there was no plaque for double recombinants and small plaques in the wild-type. The genotypes went accordingly.
The parents genotype would look like: 31 ---------X----------------------------------- 29 ----------------------------------X----------
The recombinants would look like: double mutant ---------X-----------------------X---------- Wild-type ----------------------------------------------
It is important to note the difference between recombination frequency and reversion frequency. Recombination frequency was found by dividing the titer on K by the difference between the titer on B and the titer on U and multiplying by 2. The titer on K

Related

  • Grignard Lab
    1077 words | 5 pages
  • Lab 1
    1641 words | 7 pages
  • Pendulum Lab
    918 words | 4 pages
  • Amu Scin138 Lab 9
    1573 words | 7 pages
  • lab report microbiology
    2906 words | 12 pages
  • Thermodynamics Lab
    886 words | 4 pages
  • Lab Repotrt
    1625 words | 7 pages
  • Waves Lab
    1281 words | 6 pages
  • Biology Lab
    2386 words | 10 pages
  • Lab Report
    12061 words | 49 pages