DNA EXTRACTION

1223 words 5 pages
Much can be learned from studying an organisms DNA. The first step to doing this is extracting DNA from cells. In this experiment, you will isolate DNA from the cells of fruit. Materials (1) 10 mL Graduated Cylinder(2) 100 mL Beakers15 cm Cheesecloth1 Resealable Bag1 Rubber Band (Large. Contains latex pleasewear gloves when handling if you have a latex allergy).Standing Test TubeWooden Stir StickFresh, Soft Fruit (e.g., Grapes, Strawberries, Banana, etc.) ScissorsDNA Extraction SolutionIce Cold EthanolYou Must ProvideContains sodium chloride, detergent and waterFor ice cold ethanol, store in the freezer 60 minutes before use. Procedure If you have not done so, prepare the ethanol by placing it in a freezer for approximately 60 minutes. …show more content…
However, since alcohol is non-polar, the negatively charged molecules of DNA tend to group together, forming clumps, which precipitate out of the alcohol solution. What else might be in the ethanol/aqueous interface How could you eliminate this When you precipitate the solution with cold ethanol 70 you would have a mix of DNA, RNA and some soluble salts. You should be able to centrifuge again so the DNA/RNA forms a pellet and drain the liquid out. Which DNA bases pair with each other How many hydrogen bonds are shared by each pair How is information to make proteins passed on through generations Watch the following Virtual Lab demonstrating DNA Extraction. In this experiment, how do the Lysis Solution and the Salt Solution vary by function Identify one step which was included in the Virtual Lab which was not required in the hands-on experiment. Then, identify one step which was included in the hands-on experiment, but not the virtual lab. Why werent these steps required INCLUDEPICTURE ../../../../../../../../Karuna/ESL01/2014/2920March/Production/Lab2013/CourseRoot/images/lab013banner01.jpg MERGEFORMAT INCLUDEPICTURE ../../../../../../../../Karuna/ESL01/2014/2920March/Production/Lab2013/CourseRoot/images/lab013banner02.jpg MERGEFORMAT 2013 eScience Labs, LLC.All Rights Reserved HYPERLINK http//www.esciencelabs.com t _blank INCLUDEPICTURE ../../../../../../../../Karuna/ESL01/2014/1820March/Production/Lab03/CourseRoot/images/esl_logo.jpg MERGEFORMAT

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