The Specificity of Albumin Binding Experiment

1289 words 6 pages
The specificity of albumin binding experiment was to determine the binding interactions that occur between serum albumin and three synthetic dyes with the use of electrophoretic procedure. Whole blood, or plasma. Clots upon standing and if the clot is removed, the remaining straw colored fluid is called serum. The major protein in serum is albumin which functions as a carrier molecule for the transport of certain small molecular weight compounds in blood. Molecules that bind to serum albumin are fatty acids, hormones and some synthetic dyes. In this experiment the synthetic dyes used are Bromophenol Blue, Ponceau S and Orange G. we observed that free dyes not bound to albumin migrate faster that albumin or dyes bound to albumin. This …show more content…
Group six didn’t work for us because of human error. The mixture was done wrongly.
Groups 5 and 2
Group 5 which looked at ligand binding is protein specific looks at two different proteins in the mixture. Group 2 looks at albumin binding is saturable and ligand specific. The mixture was done in the following order and measurements the tube number corresponds with the lane the mixture is put it.
Group 5 Tube/lane # | Buffer | Protein | Ponceau S | 1 | 20ul | 0 | 5ul | 2 | 10ul | 10ul hemoglobin | 5ul | 3 | 20ul | 0 | 5ul | 4 | 10ul | 10ul BSA | |

Group 2 Tube/lane # | Buffer | BSA | Ponceau S | 1 | 20ul | 0ul | 10ul | 2 | 15ul | 10ul | 5ul | 3 | 10ul | 10ul | 10ul | 4 | 0ul | 10ul | 20ul |

Fig2. The 1st picture on the left shows Andersons results of group5, the 1st half on the 2nd picture with the big arrow shows our results, the 2nd half of the picture is the experimental results of group 2 and the last picture is Andersons result of group 2.
Fig2. The 1st picture on the left shows Andersons results of group5, the 1st half on the 2nd picture with the big arrow shows our results, the 2nd half of the picture is the experimental results of group 2 and the last picture is Andersons result of group 2.

In group 5 of andersons experiment, there was no binding in the mixture that contained the hemoglobin, there was binding in the mixture that contained albumin. The rrest of the

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