The Effect of Enzyme Concentration on Rate of Reaction

2616 words 11 pages

The experiment was carried out to investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills, the experiment was done in order to determine how the rate of reaction will be altered, whether it will increase, decrease or remain constant when the different concentration of enzymes added.


Enzymes are produced naturally in plant, animal, and microbial cell. There are thousands of different enzyme can be found in any cell. Enzymes can be describe as molecules that are greatly increase the rate of chemical reaction without themselves being changed at the end of the reaction. A chemical reaction involves the conversion of a substrate
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It is an aqueous solution consisting of a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid.
Buffer solution is needed to keep the correct pH for enzymes in many organisms to work effectively. This is because many enzymes work only under the correct end precise condition. Hence, if the pH is too far out of margin, the enzymes will slow up or stop working and denatured, thus, will cause the enzyme activity become disabled. Buffer solutions are commonly used in industrial sector such as in fermentation process and in setting the correct conditions for dye used in coloring fabrics. Some of them are also used as calibration mark for pH meters.

There is a clear positive correlation between the concentration of enzyme and the rate of reaction. Increase in enzyme concentration results in increase in the rate of reaction up to a point where all substrate has occupied with the enzyme.

Manipulated variable: Concentration of enzyme
Responding variable: Volume of oxygen produced
Fixed variables: pH, temperature, concentration of substrate


• 250 ml beaker • 50 ml measuring cylinder • 5 ml measuring cylinder • Dropper • Bung • Conical flask • Test tube • Spatula


• Blended potato • pH 6.5 buffer solution • Hydrogen peroxide • Tap water


1. All the apparatus was rinsed with tap water to remove any foreign substances.

2. A


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