Laboratory Assignment: Virtual Microscopy

1617 words 7 pages
BIOL 1020
Lab Assignment: Virtual Microscopy
Start by re-saving this file as follows: lab_surname_virtualmicroscopy.rtf, substituting your own surname.
Type your responses to the questions below where indicated. Remember to save your work frequently.
Viewing Magnification, Field of View, and Specimen Size
1. On the UD Virtual Compound Microscope, examine the onion root tip slide at 400X viewing magnification. Ensure that the image is centred.
What is the approximate size of an onion root tip cell? Show your work and specify which dimension of the cell (length or width) you are using. (2 marks) RESPONSE: The dimension of the cell I am using is length. The number of specimen is 11.
The Field of view would be 0.35 mm because we
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The actual size for the longest part of the paper is 28cm and the size of organism
C when measured is 2.3cm.
2.3cm/13.4cm=X/28cm
0.172cm=X/28cm
X=4.80cm
To find the scale bar:
1.2/13.4cm(28cm)=X
X=0.251cm
0.251cm x 10cm=25.1cm
X=25.1mm/48.0mm(0.55)
X= 0.288mm is the actual size of the scale bar.
PLEASE LEAVE THE SPACE BELOW EMPTY FOR TA COMMENTS

Interpreting Micrographs
The questions below refer to the images on the Interpreting Micrographs page of the Virtual Microscopy Lab.
8. Micrograph A shows the phospholipid bilayer of a plasma membrane.
Estimate the width of the bilayer, and explain how you did so. (1 mark)
RESPONSE: Using equations and method in question 7 I would have to say that the phospholipid bilayer would be 0.67 microns.
PLEASE LEAVE THE SPACE BELOW EMPTY FOR TA COMMENTS

9. Micrograph B shows two cells of the same type treated with fluorescent stains. Assuming that the outer perimeter of the image where the stain ends is in fact the outer boundary of the cells themselves, what type of cells do you think these are - plant, animal, or bacterial? How did you rule out the other two options? (1 mark)
RESPONSE: I believe that these two cells are animal cells because one bacterial cells are prokaryotic and not eukaryotic which means that it does not contain a nucleus so that takes bacterial out of the picture. It also wouldn’t be a plant cell because plant

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